Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803

We have performed the first comparative analysis of the potential of two physiologically-diverse model cyanobacteria, Synechococcus PCC 7002 (S.7002) and Synechococcus elongatus PCC 7942 (S.7942), for the photosynthetic production of four chemically-different high-value terpenes: two monoterpenes limonene and pinene, and two sesquiterpenes bisabolene and farnesene. We showed, for the first time, that S.7002 and S.7942 can produce farnesene and bisabolene, respectively. Both cyanobacteria produced farnesene (S.7942 produced more efficiently than S.7002) more efficiently than the other tested terpenes (especially pinene, the weakest produced terpene). S.7002 produced limonene more efficiently than bisabolene, whereas S.7942 produced bisabolene more efficiently than limonene. These findings suggest that S.7942 is better suited to produce sesquiterpenes than monoterpenes. Interestingly, higher levels of terpenes were produced by S.7942 and S.7002 expressing a terpene-synthase gene from both an RSF1010-derived replicating plasmid and a neutral chromosomal site, as compared to either the plasmid alone or the chromosome alone. These results suggest that in both cyanobacteria, the production of terpenes is more limited by the activity of terpene synthases than the abundance of terpene precursors. Finally, higher levels of terpenes were produced by S.7002 growing on urea (a frequent pollutant) as compared to nitrate or ammonium, the standard nitrogen sources for cyanobacteria.


Introduction
Terpenes are a large family of chemicals with the formula (C 5 H 8 ) n that are often produced by plants where they act in their photoautotrophic metabolism, protection against pathogens, and/or attraction of pollinators. Many high-value terpenes are exploited by cosmetic, food, pharmaceutical, and/or biofuel industries, including bisabolene, farnesene, limonene, and pinene, the focus of the present study [1,2].
All terpenes are derived from the five-carbons (C5) building blocks, isopentenyl pyrophosphate (IPP), and dimethylallyl pyrophosphate (DMAPP) that are condensed to form the geranyl pyrophosphate (GPP, C10) precursor of monoterpenes (C 10 H 16 ) such as limonene and pinene. The subsequent addition of one IPP unit on GPP forms the farnesyl pyrophosphate (FPP, C15) precursor of sesquiterpenes (C 15 H 24 ), such as bisabolene and farnesene ( Figure 1). Instead of plants that must be preserved for food production, cyanobacteria, the robust photosynthetic prokaryotes that colonize our planet, can be used for the sustainable production of terpenes from the plentiful natural resources: solar energy, water, CO 2, and minerals [1]. Cyanobacteria possess the methylerythritol-4-phosphate (MEP) pathway that produces GPP and FPP (Figure 1), which can be transformed into terpenes. For this purpose, synthetic genes encoding terpene synthases need to be adapted to the cyanobacterial codon usage and subsequently introduced and expressed in cyanobacteria [1,2].
The model (unicellular) cyanobacteria Synechococcus PCC 7002 (hereafter S.7002) and Synechococcus elongatus PCC 7942 (S.7942) are attractive for this purpose, in having good genetic tools and interesting physiological differences (for review, see [3]) which make their comparative analysis very interesting. The salt-tolerant marine model S.7002 (genome size 3.41 Mb) is able to grow on nitrate (the classical nitrogen source for cyanobacteria) or urea (cheaper than nitrate) [4], but it requires vitamin B12 for growing [5]. In contrast, the salt-sensitive freshwater model S.7942 (genome size 2.74 Mb) does not require vitamin B12 but is unable to grow on urea [6]. S.7002 and S.7942 have other key differences in their DNA repair [7] and glutathione-dependent systems that play a prominent role in tolerance to stresses [3].
Two proof-of-concept studies showed that S.7002 can be engineered for the photosynthetic production of bisabolene and limonene [8] or pinene [9] but not farnesene, as we presently report. Concerning S.7942, several laboratories reported that S.7942 can be engineered for the production of farnesene [10][11][12] or limonene [13,14], but neither bisabolene nor pinene, as we report here. The limited number of previous studies and their multiple experimental differences (nature of the terpene synthase genes and their promoters, and of the chromosome/plasmid vectors propagating them) make it difficult to know whether S.7002 and/or S.7942 are suitable chassis for the photoproduction of chemically-diverse terpenes or only a few of them.
In this study, we have performed the first comparative analysis of the potential of the two physiologically-diverse model cyanobacteria, S.7002 and S.7942, for the photosynthetic production of four chemically-different high-value terpenes. Practically, we have employed the same genetic strategy to engineer S.7002 and S.7942 for the photoproduction of two monoterpenes, limonene (cyclic molecule) and pinene (bicyclic), and two sesquiterpenes, bisabolene (cyclic) and farnesene (linear), in order to rationally compare the potential of these two model cyanobacteria for the production of diverse terpenes. Each terpene synthase gene was expressed from the same strong promoter propagated by the same autonomously replicating plasmid vector in both S.7002 and S.7942. We showed for the first time that S.7002 and S.7942 can produce farnesene and bisabolene, respectively. Both S.7002 and S.7942 produced farnesene and pinene with the best (farnesene) and the lowest (pinene) efficiencies. Furthermore, S.7002 produced limonene better than bisabolene, whereas S.7942 produced bisabolene more efficiently than limonene. We also showed for the first time that S.7002 cultivated on urea (a frequent pollutant) produces terpenes more efficiently than cultures growing on either nitrate or ammonium (standard but more expensive nitrogen sources for cyanobacteria). Finally, we showed in both S.7002 and S.7942 that increasing the copy number of the terpene synthase-encoding gene increases the level of terpene production, suggesting that terpene production is more limited by the activity of terpene synthases rather than the abundance of terpene precursors.

Bacterial Strains and Growth Conditions
E. coli strains used for gene manipulations (TOP10 and NEB10 beta, Table S1) or conjugative transfer of pC-derived replicative plasmids (Table S1) to S.7942 or S.7002 (CM404 [15]) were grown at 37 • C (TOP10 and NEB10 beta) or 30 • C (CM404) on LB  [17] for S.7942, or A+ containing B12 vitamin (4 µg·L −1 ) for S.7002 [18]. The terpene-producing strains were grown in the presence of the selective antibiotics: Km 50 µg·mL −1 for both S.7002 and S.7942, and/or Sm 50 µg·mL −1 and Sp 50 µg·mL −1 for S.7002 and Sm 5 µg·mL −1 and Sp 5 µg·mL −1 for S.7942. For some experiments carried out with S.7002, the standard nitrogen source (NaNO 3 , 12 mM) was replaced by urea or ammonium. Nitrate-grown cells were washed twice and resuspended in nitrogen-free media that were supplemented with either urea or nitrate, as indicated. Growth in liquid cultures was monitored by regular measurements of optical density at 750 nm (OD 750 ) using a spectrophotometer (Jenway 6700).
The presence of the terpene-synthase-encoding DNA cassette propagated in pCderived plasmids or a neutral chromosomal site was verified by PCR and DNA sequencing (Mix2Seq Kit, Eurofins Genomics) using appropriate oligonucleotide primers (Table S2).

Terpene Collection and Quantification by Gas Chromatography-Mass Spectrometry
S.7002 and S.7942 strains engineered for terpene production were photoautotrophically grown in the presence of selective antibiotics in 250 mL erlenmeyers containing 50 mL cell suspensions overlaid with 20% (vol/vol) dodecane (analytical grade, Sigma-Aldrich) to trap terpenes [1,20,23]. At time intervals, 300 µL aliquots of the dodecane overlay were collected, and 1 µL aliquots of these dodecane samples were injected in a split mode 10:1 (limonene) or 5:1 (E-α-bisabolene, farnesene, and pinene) into a GC-MS apparatus (Trace1300 (GC) + ISQ LT (MS), ThermoScientific). The quantification of terpenes was performed as we previously described [20,23]. In this study, we tested and compared the ability of the unicellular model cyanobacteria S.7002 and S.7942 to produce the four chemically-different high-value terpenes: bisabolene, farnesene, limonene, and pinene ( Figure 1). For this purpose, we used the following terpene synthases-encoding genes that worked well in cyanobacteria [1], namely: (i) the Abies grandis E-α-bisabolene synthase (bs), (ii) the Picea abies α-farnesene synthase (fs), (iii) the Mentha spicata 4S-limonene synthase gene (ls) and (iv) the Pinus taeda (+)-α-pinene synthase gene (ps). Each codon-adapted terpene synthase gene was cloned downstream of the strong lambda-phage pR-promoter and associated cro ribosome-binding site of the autonomously replicating RSF1010-derived [15,19] pC plasmid vector [24], yielding the plasmids pCBS, pCFS, pCLS, and pCPS [20,23]. These plasmids were introduced by conjugation in S.7002 and S.7942 [15,23], yielding the strains represented in Figure 2. In each case, two independent Sm R /Sp R clones were selected and analyzed by PCR and DNA Biomolecules 2023, 13, 504 5 of 15 sequencing ( Figure S1) to verify that pCBS, pCFS, pCLS, and pCPS plasmids replicate stably in S.7002 and S.7942, without affecting their photoautotrophic growth ( Figure S2). is arbitrarily shown as attached to the cell membrane to distinguish it from the pC-derived replicative plasmids. These Sm R /Sp R plasmids, which express a terpene-synthase encoding gene from the strong λ phage pR promoter (red triangle), are named pCBS (bisabolene synthase, lightblue arrow), pCFS (farnesene synthase, green arrow), pCLS (limonene synthase, yellow arrow) and pCPS (pinene synthase, brown arrow). Note that the chromosome of two strains of both S.7002 and S.7942 harbor a pR-LS (Km R ) and a pR-FS (Km R ) gene cassette, respectively. They served for the comparison of terpene production driven by either or both the replicating plasmid and the chromosome. is arbitrarily shown as attached to the cell membrane to distinguish it from the pC-derived replicative plasmids. These Sm R /Sp R plasmids, which express a terpene-synthase encoding gene from the strong λ phage pR promoter (red triangle), are named pCBS (bisabolene synthase, light-blue arrow), pCFS (farnesene synthase, green arrow), pCLS (limonene synthase, yellow arrow) and pCPS (pinene synthase, brown arrow). Note that the chromosome of two strains of both S.7002 and S.7942 harbor a pR-LS (Km R ) and a pR-FS (Km R ) gene cassette, respectively. They served for the comparison of terpene production driven by either or both the replicating plasmid and the chromosome.

Results and Discussion
The pR-LS and pR-FS recombinant genes were also cloned in the NS 7002 and NSI 7942 neutral chromosomal sites of S.7002 and S.7942, respectively, to compare the level of terpene production driven by either or both the plasmid and the chromosome ( Figure 2).

Unlike Synechococcus PCC 7002, the Growth of Synechococcus elongatus PCC 7942 Is Affected by the Dodecane Overlay Used to Trap Terpenes, but Dodecane-Adapted Strains Grow Healthy under Dodecane
As terpenes are volatile chemicals, cyanobacteria engineered for terpene production are routinely grown under a dodecane overlay to trap terpenes, which are subsequently quantified by gas chromatography coupled with mass spectrometry [1]. The growth of S.7002 was not affected by a dodecane overlay ( Figure 3A), in agreement with previous studies on S.7002 [8,9]. In contrast, the growth of S.7942 was strongly delayed by a dodecane overlay ( Figure 3B), but S.7942 strains precultivated under dodecane grew subsequently well under dodecane ( Figure 3), a finding not discussed by previous workers [10][11][12]. Consequently, we used dodecane-adapted strains to analyze terpene production by S.7942. Cells were grown photoautotrophically in the absence or presence of a dodecane (20% vol/vol) overlay. S.7942, denoted as da (da for dodecane-adapted cultures), was inoculated with cells that were precultivated under dodecane for more than two weeks. Error bars represent standard deviations from biological triplicates.

The First Report of Farnesene Production by Synechococcus PCC 7002: the Production Is Lower than That of Synechococcus elongatus PCC 7942
The production of farnesene during the photoautotrophic growth of the S.7002 and S.7942 strains propagating the pCFS plasmid, or the empty pC plasmid vector used as a negative control, were measured over 21-day periods of photoautotrophic growths. GC-MS analysis of dodecane overlay samples from the pCFS propagating strains showed a peak with a similar retention time and ion chromatogram than a pure standard of αfarnesene, which was not observed in the negative-control pC strains ( Figures S3A and  S4A). These data showed that both S.7002 and S.7942 can photosynthetically produce farnesene ( Figure 4A), a novel finding in the case of S.7002. However, lower levels of farnesene were produced by S.7002 (∼2 mg·L −1 after 21 days, Figure 4), as compared to S.7942 (∼8 mg·L −1 after 21 days, Figure 4A).  The production of farnesene during the photoautotrophic growth of the S.7002 and S.7942 strains propagating the pCFS plasmid, or the empty pC plasmid vector used as a negative control, were measured over 21-day periods of photoautotrophic growths. GC-MS analysis of dodecane overlay samples from the pCFS propagating strains showed a peak with a similar retention time and ion chromatogram than a pure standard of α-farnesene, which was not observed in the negative-control pC strains ( Figures S3A and S4A). These data showed that both S.7002 and S.7942 can photosynthetically produce farnesene ( Figure 4A), a novel finding in the case of S.7002. However, lower levels of farnesene were produced by S.7002 (~2 mg·L −1 after 21 days, Figure 4), as compared to S.7942 (~8 mg·L −1 after 21 days, Figure 4A). produce various high-value terpenes.
The level of farnesene production driven by the pCFS plasmid in S.7942 and S.7002 could be really compared to the level driven by the same pCFS plasmid we previously reported in Synechocystis PCC 6803 (hereafter S.6803) [20], another unicellular model cyanobacterium phylogenetically-distant to both S.7942 and S7002 [3]. Interestingly, the pCFS-driven farnesene production in S.7942 and S.7002 (∼8 and ∼2 mg·L −1 after 21 days, respectively, Figure 4A) was lower than what we observed in S.6803 harboring the same pCFS plasmid (∼30 mg·L −1 after 21 days, [20], Figure S5A). These findings indicate that S.7942 and S.7002 are less suitable cyanobacterial factories than S.6803 for the photoproduction of farnesene. . Cells were grown under dodecane (20% vol/vol) in otherwise standard photoautotrophic conditions to assay terpene production for 21 days. Error bars represent standard deviation from n ≥ 2 biological replicates. Note that note that no pinene was produced by S.7942

First Report of Bisabolene Production by Synechococcus Elongatus PCC 7942: The Production is Similar to that of Synechococcus PCC 7002
The photosynthetic production of bisabolene by the S.7002 and S.7942 strains propagating the pCBS plasmid, or the empty pC plasmid, was measured over a 21-day period of cultures ( Figure 4B). GC-MS analysis of dodecane overlay samples from the pCBS strains showed a peak with a similar retention time ( Figures S3A and S4A) and ion chromatogram than a pure standard of E-α-bisabolene, which was not observed in the The level of farnesene production by S.7942 (~1.5 mg·L −1 after seven days, Figure 4A) was roughly similar to what was reported by another group (~3 mg·L −1 after eight days, no subsequent measurements) [10,12]) who cloned into a neutral chromosomal site (we used a replicative plasmid) a codon-adapted α-farnesene synthase gene from Malus domestica Borkh or Picea abies (we used only the Picea abies enzyme) expressed from the strong pTrc promoter (we used the strong lambda-phage pR promoter). However, the multiple experimental differences between these reports and our own study make their comparison difficult. This explains why we employed the same genetic engineering strategy to explore the capability of various model cyanobacteria to photosynthetically produce various high-value terpenes.
The level of farnesene production driven by the pCFS plasmid in S.7942 and S.7002 could be really compared to the level driven by the same pCFS plasmid we previously reported in Synechocystis PCC 6803 (hereafter S.6803) [20], another unicellular model cyanobacterium phylogenetically-distant to both S.7942 and S7002 [3]. Interestingly, the pCFS-driven farnesene production in S.7942 and S.7002 (~8 and~2 mg·L −1 after 21 days, respectively, Figure 4A) was lower than what we observed in S.6803 harboring the same pCFS plasmid (~30 mg·L −1 after 21 days, [20], Figure S5A). These findings indicate that S.7942 and S.7002 are less suitable cyanobacterial factories than S.6803 for the photoproduction of farnesene.

First Report of Bisabolene Production by Synechococcus Elongatus PCC 7942: The Production
Is Similar to That of Synechococcus PCC 7002 The photosynthetic production of bisabolene by the S.7002 and S.7942 strains propagating the pCBS plasmid, or the empty pC plasmid, was measured over a 21-day period of cultures ( Figure 4B). GC-MS analysis of dodecane overlay samples from the pCBS strains showed a peak with a similar retention time (Figures S3A and S4A) and ion chromatogram than a pure standard of E-α-bisabolene, which was not observed in the negative-control pC strain (Figures S3A and S4A). Both S.7002 and S.7942 produced similar levels of bisabolene (~0.5 and~2.0 mg·L −1 after 21 days, respectively, Figure 4B).
The production of bisabolene by S.7002 (~0.1 mg·L −1 after four days;~0.5 mg·L −1 after 21 days) is lower than the previously reported level (~0.5 mg·L −1 after six days, no subsequent measurement [8]) observed after cloning the Abies grandis (E)-α-bisabolene synthase gene expressed from the cpc phycocyanin promoter (we used the lambda-phage pR promoter) into a neutral chromosomal site (we used a replicative plasmid). The important differences between the previous report and our study make their comparison difficult.
The presently-observed levels of bisabolene production driven by the pCBS plasmid in S.7002 and S.7942 (~0.5 and~2.0 mg·L −1 after 21 days, respectively, as seen in Figure 4B) were lower than the value we previously reported for S.6803 harboring the same pCBS plasmid (10-14 mg·L −1 after 21 days, [20,25]). Together with the above-mentioned findings, these data ( Figure S5B) indicate that S.7942 and S.7002 are less efficient cyanobacterial factories than S.6803 for the photoproduction of the sesquiterpenes farnesene and bisabolene.

The Synechococcus PCC 7002 and Synechococcus elongatus PCC 7942 Strains Harboring the pCLS Plasmid Produce Limonene in This Order
The photosynthetic production of limonene by the S.7002 and S.7942 strains propagating the pCLS plasmid, or the empty pC plasmid, was monitored for 21 days ( Figure 4C). Dodecane overlay samples from the pCLS strains, not the pC strains, showed a GC-MS peak typical of a pure S-(-)-limonene standard ( Figures S3B and S4B). Interestingly, a higher level of limonene was produced by S.7002 (~1.0 mg·L −1 after 14 days and~1.7 mg·L −1 after 21 days, Figure 4C) as compared to S.7942 (~0.3 mg·L −1 after 14 days, Figure 4C).
The presently-observed production of limonene by S.7002 (~0.3 mg·L −1 after four days, Figure 4C) is lower than the previously reported level (~4.0 mg·L −1 after four days, but no subsequent measurement [8]) observed after cloning into a neutral chromosomal site (we used a replicative plasmid) the Mentha spicata limonene synthase gene expressed from the cpc phycocyanin promoter (we used the lambda-phage pR promoter).
Similarly, the presently-observed production of limonene by S.7942 (~0.06 mg·L −1 after seven days, Figure 4C) seems lower than the level (~17 mg·L −1 after seven days, but no subsequent measurement [14]) observed after cloning into a neutral chromosomal site (we used a replicative plasmid) the Mentha spicata limonene synthase gene expressed from the psbA promoter (we used the lambda-phage pR promoter).
The multiple experimental differences between the previous reports and our study make it difficult to ascribe the difference in limonene production to one or several parameters: growth conditions, nature of the promoter expressing the limonene synthase gene, and/or of the cloning vehicle propagating it.
The presently-observed level of limonene production driven by the pCLS plasmid in S.7002 (~1.7 mg·L −1 after 21 days, Figure 4C) is slightly higher than the value we previously observed in S.6803 (~0.7 mg·L −1 after 21 days [20,25]. These findings ( Figure S5C) indicate that S.7002 is a cyanobacterial cell factory as effective as S.6803 for the production of the monoterpene limonene, unlike what we observed in the case of the two sesquiterpenes: bisabolene and farnesene ( Figure S5B,C).

The Synechococcus PCC 7002 and Synechococcus elongatus PCC 7942 Strains Harboring the pCPS Plasmid Produce Pinene Inefficiently
The photosynthetic production of pinene by the S.7002 and S.7942 strains propagating the pCPS plasmid, or the empty pC plasmid, was measured for 21 days ( Figure 4D). GC-MS analysis of dodecane overlay samples from the pCPS strain of S.7002 showed a peak typical of a pure standard of α-pinene, which was not observed in the pC strain ( Figure S3C). In contrast, no pinene production was detected in S.7942 harboring pCPS ( Figure 4D).
The presently observed production of pinene by S.7002 (~60 µg·L −1 after 21 days) is lower than the previously reported level (1.5 mg·L −1 after six days, [9]) observed after cloning into the endogenous pAQ1 plasmid (we used the non-cyanobacterial pC vector derived from the broad-host-range plasmid RSF1010 [20]) a Abies grandis gene encoding a 6xhistidine-tagged pinene synthase (we used the native Pinus taeda enzyme) expressed from the cpc promoter (we used the lambda-phage pR promoter). As both the pAQ1 and pC vectors, on the one hand, and the cpc and pR promoters, on the other hand, function well in cyanobacteria [3], we assume that the 6xhistidine-tagged pinene synthase from Abies grandis is more efficient than the native Pinus taeda enzyme we have used in this study ( Figure 4D), and our previous analysis of pinene production in S.6803 [20], Figure S5D).

Comparison of Limonene Production in Synechococcus PCC 7002 Driven by the Recombinant LS Gene Propagated by Either or Both the pCLS Replicative Plasmid and the NS 7002 Neutral Chromosomal Site
Because the activity of an enzyme can be improved by increasing the copy number of the gene encoding it, we attempted to increase the limonene production of the S.7002 strain harboring pCLS by cloning an extra copy of the same pR-LS recombinant gene (LS expressed from the strong pR promoter) in the NS 7002 neutral chromosomal site (a region in between A0935-A0936 [26]) often used for gene cloning in S.7002 [3]. Therefore, a pR-LS-Km R DNA cassette was assembled by Gibson [27] (Figure S6) in between the two 500-bp DNA regions surrounding NS 7002 that serve as a platform of homology for DNA recombination promoting targeted integration of the pR-LS-Km R cartridge into the chromosome. The resulting pTwist_NS-S7002p R LSKm R plasmid (Table S1) was transformed to S.7002 pCLS cells, where the pR-LS-Km R DNA cassette was integrated into all chromosome copies ( Figure S7). The resulting strain expressing the pR-LS gene from both the pCLS plasmid and the chromosome was designated as pCLS + chrLS ( Figure 2).
In parallel, the pTwist_NS-S7002p R LSKm R plasmid was transformed to S.7002 wildtype cells, where the pR-LS-Km R DNA cassette was integrated into all chromosome copies ( Figure S7). The resulting strain named chrLS served to measure the level of limonene production ( Figure 2) driven by the chromosomal pR-LS gene.
All three strains, pCLS, chrLS, and pCLS + chrLS, grew well under standard photoautotrophic conditions in the presence of the dodecane overlay ( Figure 5). Interestingly, the level of limonene produced by the pCLS + chrLS strain was roughly equivalent to the sum of limonene produced by the strains pCLS and chrLS, which were similarly active ( Figure 5). These results indicate that the production of terpenes by an engineered S.7002 strain is probably more limited by the activity of the employed terpene synthase than the abundance of terpene precursors, as we recently discussed in the case of S.6803 [20].

Comparison of Farnesene Production in Synechococcus Elongatus PCC 7942 Driven by the Recombinant FS Gene Propagated by Either or Both the pCFS Replicative Plasmid and the NSI7942 Neutral Chromosomal Site
We also attempted to increase farnesene production in the pCFS S.7942 strain by cloning an extra copy of the pR-FS recombinant gene (FS expressed from the pR promoter) in the NSI7942 neutral chromosomal site (GenBank accession U30252, [28]) a site often used for gene cloning in S.7942 [3]. Therefore, a pR-FS-Km R DNA cassette was assembled by Gibson [27] (Figure S6) in between the two 300-bp DNA regions surrounding NSI7942 to mediate the targeted integration of the pR-FS-Km R cartridge in NSI7942. The resulting pTwist_NSI-S7942 pRFSKm R plasmid (Table S1) was transformed to S.7942 pCFS cells where the pR-FS-Km R DNA cassette was integrated into the NSI7942 site of all chromosome copies ( Figure S8). The resulting strain expressing the pR-FS gene from both the pCFS plasmid and the chromosome was designated as pCFS + chrFS ( Figure 2).
In parallel, the pTwist_NSI-S7942 pRFSKm R plasmid was transformed to S.7942 wildtype cells where the pR-FS-Km R DNA cassette was integrated into the NSI7942 site of all chromosome copies ( Figure S8). The resulting strain named chrFS ( Figure 2) served to measure the level of farnesene production driven by the chromosomal pR-FS gene ( Figure  6).
The two pCFS and chrFS grew slightly better than the pCFS + chrFS strain under standard photoautotrophic conditions, irrespective of the presence of the terpenetrapping dodecane overlay ( Figure 6A). Interestingly, the level of farnesene produced by the pCFS + chrFS strain was roughly equivalent to the sum of farnesene produced by the strains pFCS and chrFS, which were similarly active ( Figure 6B). These results indicate that the production of terpenes by an engineered S.7942 strain is probably more limited by the terpene-synthase activity than the terpene-precursors abundance, as we recently discussed in the case of S.6803 [20]. This assumption is consistent with the finding that terpene synthases are slow enzymes and that selecting a terpene synthase with high Kcat and low Km is pivotal in terpenoid biosynthesis [29].

Comparison of Farnesene Production in Synechococcus Elongatus PCC 7942 Driven by the Recombinant FS Gene Propagated by Either or Both the pCFS Replicative Plasmid and the NSI 7942 Neutral Chromosomal Site
We also attempted to increase farnesene production in the pCFS S.7942 strain by cloning an extra copy of the pR-FS recombinant gene (FS expressed from the pR promoter) in the NSI 7942 neutral chromosomal site (GenBank accession U30252, [28]) a site often used for gene cloning in S.7942 [3]. Therefore, a pR-FS-Km R DNA cassette was assembled by Gibson [27] (Figure S6) in between the two 300-bp DNA regions surrounding NSI 7942 to mediate the targeted integration of the pR-FS-Km R cartridge in NSI 7942 . The resulting pTwist_NSI-S7942 p R FSKm R plasmid (Table S1) was transformed to S.7942 pCFS cells where the pR-FS-Km R DNA cassette was integrated into the NSI 7942 site of all chromosome copies ( Figure S8). The resulting strain expressing the pR-FS gene from both the pCFS plasmid and the chromosome was designated as pCFS + chrFS ( Figure 2).
In parallel, the pTwist_NSI-S7942 p R FSKm R plasmid was transformed to S.7942 wildtype cells where the pR-FS-Km R DNA cassette was integrated into the NSI 7942 site of all chromosome copies ( Figure S8). The resulting strain named chrFS ( Figure 2) served to measure the level of farnesene production driven by the chromosomal pR-FS gene ( Figure 6).
The two pCFS and chrFS grew slightly better than the pCFS + chrFS strain under standard photoautotrophic conditions, irrespective of the presence of the terpene-trapping dodecane overlay ( Figure 6A). Interestingly, the level of farnesene produced by the pCFS + chrFS strain was roughly equivalent to the sum of farnesene produced by the strains pFCS and chrFS, which were similarly active ( Figure 6B). These results indicate that the production of terpenes by an engineered S.7942 strain is probably more limited by the terpene-synthase activity than the terpene-precursors abundance, as we recently discussed in the case of S.6803 [20]. This assumption is consistent with the finding that terpene synthases are slow enzymes and that selecting a terpene synthase with high Kcat and low Km is pivotal in terpenoid biosynthesis [29].

Influence of Growth Conditions on Terpene Production: First Report that Synechococcus PCC 7002 Cultivated on Urea Produces Terpenes More Efficiently than Cultures Growing on Nitrate or Ammonium in This Order
In the view of reducing the costs of future industrial production of terpenes, eventually coupled with wastewater treatment, we have tested the influence of urea (frequently present in natural waters [4]) on cell growth and the production of the monoterpene limonene and the sesquiterpene farnesene. For this purpose, we have used S.7002 because it grows well on all three nitrogen sources [5], ammonium, nitrate (the standard nitrogen source for cyanobacteria [6]), and urea (cheaper than nitrate), unlike S.7942, which cannot grow on urea because it has neither urea transport (UrtABCDE) nor catabolic (UreABCDEF) enzymes [4]. In these assays, S.7002 strains harboring the pFS or pCLS plasmids were cultivated in the same standard concentration of nitrogen (12 mM) supplied as either nitrate (NaNO3, 12 mM), ammonium (NH4Cl, 12 mM) or urea (CO(NH2)2, 6 mM). In the case of urea (and nitrate as a control), we tested both protocols in which nitrogen is supplied either all at once, at the beginning of the culture, or provided gradually as successive doses during cultivation as was required by the other cyanobacteria Cyanothece PCC 7425 and Synechocystis PCC 6803 [20,23]. The results ( Figure  7) showed, for the first time, that S.7002 growing on urea as the sole nitrogen source can produce terpene more efficiently than cells cultivated on nitrate or ammonium (in this order). Interestingly, Cyanothece PCC 7425 and S.6803 produced terpene less efficiently (C.7425) or similarly (S.6803) when cultivated on urea as compared to nitrate [20,23].

Influence of Growth Conditions on Terpene Production: First Report That Synechococcus PCC 7002 Cultivated on Urea Produces Terpenes More Efficiently Than Cultures Growing on Nitrate or Ammonium in This Order
In the view of reducing the costs of future industrial production of terpenes, eventually coupled with wastewater treatment, we have tested the influence of urea (frequently present in natural waters [4]) on cell growth and the production of the monoterpene limonene and the sesquiterpene farnesene. For this purpose, we have used S.7002 because it grows well on all three nitrogen sources [5], ammonium, nitrate (the standard nitrogen source for cyanobacteria [6]), and urea (cheaper than nitrate), unlike S.7942, which cannot grow on urea because it has neither urea transport (UrtABCDE) nor catabolic (UreABCDEF) enzymes [4]. In these assays, S.7002 strains harboring the pFS or pCLS plasmids were cultivated in the same standard concentration of nitrogen (12 mM) supplied as either nitrate (NaNO 3 , 12 mM), ammonium (NH 4 Cl, 12 mM) or urea (CO(NH 2 ) 2 , 6 mM). In the case of urea (and nitrate as a control), we tested both protocols in which nitrogen is supplied either all at once, at the beginning of the culture, or provided gradually as successive doses during cultivation as was required by the other cyanobacteria Cyanothece PCC 7425 and Synechocystis PCC 6803 [20,23]. The results (Figure 7) showed, for the first time, that S.7002 growing on urea as the sole nitrogen source can produce terpene more efficiently than cells cultivated on nitrate or ammonium (in this order). Interestingly, Cyanothece PCC 7425 and S.6803 produced terpene less efficiently (C.7425) or similarly (S.6803) when cultivated on urea as compared to nitrate [20,23].

Conclusions
We have performed the first rational and comparative analysis of the ability of two physiologically-diverse model unicellular cyanobacteria, Synechococcus PCC 7002 (a marine host) and Synechococcus elongatus PCC 7942 (a freshwater host), to photosyntheti-cally produce chemically-diverse terpenes of high-value (bisabolene, farnesene, limonene, and pinene). For this purpose, each terpene synthase gene was expressed from the same strong promoter and propagated by the same RSF1010-derived replicative plasmid vector. We showed, for the first time, that S.7002 and S.7942 can be engineered for the photoproduction of farnesene and bisabolene, respectively. S.7942 and S.7002 (in this order) produced farnesene more efficiently than the other tested terpenes, especially pinene, the weakest produced terpene, similar to what we previously observed in the other model cyanobacterium Synechocystis PCC 6803 (S.6803) [20]. These findings indicate that the Pinus taeda pinene synthase does not work well in cyanobacteria. Furthermore, S.7002 produced limonene more efficiently than bisabolene, whereas S.7942 produced bisabolene more efficiently than limonene. We showed for the first time that S.7002 can produce terpenes when growing on urea (a frequent pollutant) as the sole nitrogen source, similar to what we observed for S.6803 [20]. In the case of S.7002, higher levels of terpenes were produced by cultures growing on urea, as compared to nitrate or ammonium, the standard or frequent nitrogen source for cyanobacteria, respectively. By contrast, neither S.7942 nor the fastest growing strain, Synechococcus elongatus UTEX 2973, can be used for chemical production from urea because they both lack all urea transport and catabolism enzymes [4]. Interestingly, higher levels of terpenes were produced by both S.7002 and S.7942 expressing the terpene synthase gene from both an RSF1010-derived replicating pC plasmid and a neutral chromosomal site, as we observed in Synechocystis PCC 6803 [20]. These results suggest that the production of terpenes in cyanobacteria is more limited by the activity of terpene synthases than the abundance of terpene precursors, similar to what we discussed in the case of S.6803 [20]. Finally, we compared the levels of terpene production by S.7002 and S.7942 with what we previously observed for S.6803, which was engineered in the same way as S.7002 and S.7942 [20]. We found that S.6803 produces higher levels of the sesquiterpenes farnesene and bisabolene than S7942 and S.7002 (in this order), suggesting that S.6803 could be a better cyanobacterial chassis for future industrial photoproduction of these chemicals.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/biom13030504/s1. Figure S1: PCR verification of the structure of the pC plasmid family replicating in Synechococcus PCC 7002 and Synechococcus PCC 7942. Figure S2: Influence of dodecane and pC-derived plasmids on the photoautotrophic growth of of Synechococcus PCC 7002. Figure S3A: GC-MS analyses of the dodecane overlays of cultures of the Synechoccocus PCC 7002 strains harboring the plasmids pC, pCBS or pCFS. Figure S3B: GC-MS analyses of the dodecane overlay of cultures of the Synechococcus PCC 7002 strains harboring the plasmids pC or pCLS. Figure S3C: GC-MS analyses of the dodecane overlay of cultures of the Synechococcocus PCC 7002 strains harboring the pC or pCPS plasmids. Figure S4A: GC-MS analyses of the dodecane overlays of cultures of the Synechoccocus PCC 7942 strains harboring the plasmids pC, pCBS or pCFS. Figure S4B. GC-MS analyses of the dodecane overlay of cultures of the Synechococcus PCC 7942 strains harboring the pC or pCLS plasmids. Figure S5: Comparison of the photosynthetic production of terpenes by the Synechococcus PCC 7002, Synechococcus PCC 7949 and Synechocystis PCC 6803 strains we have engineered in this study (S7002 and S7942), or a previous one (S6803). Figure S6: Construction of the pR-TPS-Km R DNA cassette for targeted insertion of a terpene synthase genes (LS or FS) in the neutral chromosome sites of Synechococcus PCC 7002 (NS 7002 ) or Synechococcus PCC 7942 (NSI 7942 ). Figure S7: PCR verification of the presence of the pR-LS-Km R cassette inserted inside the neutral chromosomal site (NS 7002 ) of Synechococcus PCC 7002. Figure S8: PCR verification of the presence of the farnesene synthase gene propagated by either or both the pCFS plasmid or the NSI 7942 neutral chromosomal site of Synechococcus PCC 7942. Table S1: Characteristics of the bacterial strains and plasmids used in this study. Table S2: Characteristics of the primers used in this study.